Journal: Science Advances
Article Title: Shear flow patterns antimicrobial gradients across bacterial populations
doi: 10.1126/sciadv.ads5005
Figure Lengend Snippet: ( A ) Experiments measuring P. aeruginosa growth for 2 hours in M9 minimal media with 16 μM H 2 O 2 at various shear rates. ( B ) Experiments measuring P. aeruginosa growth for 4 hours in LB media with gentamicin (100 μg/ml) at various shear rates. ( C ) Experiments measuring P. aeruginosa growth for 4 hours in LB media with carbenicillin (30 μg/ml) at various shear rates. Higher shear rates increase the effectiveness of H 2 O 2 , gentamicin, and carbenicillin against resistant P. aeruginosa strains. We interpret that growth gradients for gentamicin and carbenicillin have flatter slopes than H 2 O 2 because H 2 O 2 is smaller and has a higher diffusion coefficient. All experiments were performed in 27-cm–long channels. For each biological replicate, up to 30 cells were chosen at random for quantification. Shaded regions show SD of three biological replicates.
Article Snippet: Carbenicillin stock solutions were prepared from carbenicillin disodium salt (Gold Biotechnology) dissolved in water.
Techniques: Shear, Diffusion-based Assay